cole1 origins (New England Biolabs)
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 
97
Structured Review
New England Biolabs
cole1 origins
Cole1 Origins, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cole1+origins/SapI/bio_rxiv__2025__02__03__636306-163-18-22
Average 97 stars, based on 935 article reviews
Cole1 Origins, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 935 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cole1+origins/SapI/bio_rxiv__2025__02__03__636306-163-18-22
Average 97 stars, based on 935 article reviews
cole1 origins - by Bioz Stars,
2026-09
97/100 stars
Images
1) Product Images from "Generating functional plasmid origins with OriGen"
Article Title: Generating functional plasmid origins with OriGen
Journal: bioRxiv
doi: 10.1101/2025.02.03.636306
Figure Legend Snippet: a , Experimental workflow for validating generated origins. b , Assessment of ColE1-type origin function in E. coli . Matched random sequences contain the same number of mutations relative to wild-type as their corresponding OriGen-generated sequences. Lighter green indicates a single nucleotide polymorphism or single nucleotide indel was detected after sequencing. Stars represent unsuccessful experiments, which are in the process of being rerun . c , RNAI secondary structure comparison of one OriGen-generated ColE1 example vs closest WT vs matched random mutant control. d , Assessment of uncharacterized Rep3-type origin function in E. coli . e , Distribution of known motifs in examples of Rep3 OriGen-generated origins vs closest WT vs matched random mutant. In both examples, the model-generated origins led to replication while the random mutants did not.
Techniques Used: Generated, Sequencing, Comparison, Mutagenesis, Control
Related Articles
Generated:Article Title: Generating functional plasmid origins with OriGen Article Snippet: mutation was introduced into the Rep dropout cassette vector using site-directed mutagenesis. .. Golden Gate assemblies were performed using type IIS restriction enzymes BsaI-HF®v2 ( Sequencing:Article Title: Generating functional plasmid origins with OriGen Article Snippet: mutation was introduced into the Rep dropout cassette vector using site-directed mutagenesis. .. Golden Gate assemblies were performed using type IIS restriction enzymes BsaI-HF®v2 ( Comparison:Article Title: Generating functional plasmid origins with OriGen Article Snippet: mutation was introduced into the Rep dropout cassette vector using site-directed mutagenesis. .. Golden Gate assemblies were performed using type IIS restriction enzymes BsaI-HF®v2 ( Mutagenesis:Article Title: Generating functional plasmid origins with OriGen Article Snippet: mutation was introduced into the Rep dropout cassette vector using site-directed mutagenesis. .. Golden Gate assemblies were performed using type IIS restriction enzymes BsaI-HF®v2 ( Control:Article Title: Generating functional plasmid origins with OriGen Article Snippet: mutation was introduced into the Rep dropout cassette vector using site-directed mutagenesis. .. Golden Gate assemblies were performed using type IIS restriction enzymes BsaI-HF®v2 ( Synthesized:Article Title: Generating functional plasmid origins with OriGen Article Snippet: mutation was introduced into the Rep dropout cassette vector using site-directed mutagenesis. .. Golden Gate assemblies were performed using type IIS restriction enzymes BsaI-HF®v2 ( |
